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. 2016 Dec 2;11:3031–3042. doi: 10.2147/COPD.S120849

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© 2016 Wu et al. This work is published and licensed by Dove Medical Press Limited

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CSE increases PlGF expression and secretion in 16-HBE cells.

Notes: Cells were treated with or without CSE at the designed concentrations and time intervals. Media samples and cells were collected and the mRNA, protein and secretion of PlGF was assessed by qPCR (A and B), Western blot (C and D) and ELISA (E and F). Cell viability was assessed by CCK-8 assay (G). All experiments were repeated in triplicate. *P<0.05.

Abbreviations: CSE, cigarette smoke extract; PlGF, placental growth factor; HBE, human bronchial epithelium; qPCR, quantitative polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; CCK, Cell Counting Kit-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; CTL, control.

CSE increases PlGF expression and secretion in 16-HBE cells.

Notes: Cells were treated with or without CSE at the designed concentrations and time intervals. Media samples and cells were collected and the mRNA, protein and secretion of PlGF was assessed by qPCR (A and B), Western blot (C and D) and ELISA (E and F). Cell viability was assessed by CCK-8 assay (G). All experiments were repeated in triplicate. *P<0.05.

Abbreviations: CSE, cigarette smoke extract; PlGF, placental growth factor; HBE, human bronchial epithelium; qPCR, quantitative polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; CCK, Cell Counting Kit-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; CTL, control.