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. 2016 Dec 2;11:3031–3042. doi: 10.2147/COPD.S120849

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© 2016 Wu et al. This work is published and licensed by Dove Medical Press Limited

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CSE increases of Egr-1 expression, nuclear accumulation and transcriptional activity in 16-HBE cells.

Notes: Cells were treated with or without CSE at the designed concentrations (3%, 6%, 9%, 12%) and time intervals (1 h, 2 h, 4 h, 8 h, 16 h). Total RNA or whole cell lysates were collected, and the expression of Egr-1 was assessed by qPCR (A and B) and Western blot (C and D). Nuclear localization of Egr-1 was examined by immunofluorescence staining (E). Dose–response of the transcriptional activity of Egr-1 was assayed with nuclear extracts using the BD Mercury TransFactor kit (F). All experiments were repeated in triplicate. *P<0.05.

Abbreviations: CSE, cigarette smoke extract; Egr-1, early growth response-1; HBE, human bronchial epithelium; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; CTL, control.