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. 2016 Dec 2;11:3031–3042. doi: 10.2147/COPD.S120849

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© 2016 Wu et al. This work is published and licensed by Dove Medical Press Limited

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CSE increases the phosphorylation and nuclear translocation of the ERK-1/2 in 16-HBE cells.

Notes: Cells were exposed to CSE (0–240 min), and cell lysates was subjected to Western blot analysis for ERK-1/2 phosphorylation measurement (A and B). Nuclear accumulation of phospho-ERK-1/2 in CSE-treated cells (0–60 min) was detected by immunofluorescence staining (C). Cells were pretreated with U0126 (10 μmol/L, 1 h) and then CSE was added (4 h). Egr-1 and PlGF levels were examined by Western blot analysis (D). All experiments were repeated in triplicate. *P<0.05 compared with normal control; ^#P<0.05 compared with CSE treated control.

Abbreviations: CSE, cigarette smoke extract; ERK-1/2, extracellular signal-regulated kinase1/2; HBE, human bronchial epithelium; Egr-1, early growth response-1; PlGF, placental growth factor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; CTL, control.