Table 5. Summary of total and validated bioinformatic SNP calls among the sequenced accessions that included both diploids and tetraploids using allele-specific discrimination assays.
| Genotype | Diploids | Tetraploids | Total | |
|---|---|---|---|---|
| Homozygous | 122 | 360 | 482 | Total SNP callsb |
| Heterozygousa | — | 300 | 300 | |
| Total | 122 | 660 | 782 | |
| Homozygous | 108 | 319 | 427 | Validated SNP callsb |
| Heterozygous | — | 209 | 209 | |
| Total | 108 | 528 | 636 | |
| % matched calls | 88.52 | 80.00 | 81.32 |
a
Heterozygous SNP calls for tetraploids are likely the result of the homeologous SNPs derived from two sub-genomes and do not indicate that genotypes are in heterozygous state.
b
Total SNP calls and validated SNP calls are presented in File S3.