Figure 1.

Schematic representation of GATOR1 mutants generated using CRISPR/Cas9 genome editing. To generate the nprl2 and iml1 deletion mutants, two gRNAs that target the 5′ and 3′ of each gene were used. (A) nprl2 encodes a transcript of 1707 bp and a protein of 412 amino acids (FBgn0030800). The nprl2¹ deletion contains 30 amino acids from the N-terminus and four frame-shifted amino acids from the C-terminus. (B) iml1 encodes five transcripts (FBgn0035227). These transcripts encode proteins of 1471, 1472, 1503, 1511, and 1544 amino acids, respectively. The iml1¹ deletion contains 17 amino acids from the N-terminus and 53 amino acids from the C-terminus of all iml1 transcripts. Notably, the intron of iml1 also encodes a transcript Ir62a, which is also deleted. CDS, coding sequences; CRISPR, clustered regularly interspaced short palindromic repeats; gRNA, guide RNA; UTR, untranslated region.