Figure 4.

H2AK5 is an acetylation target of MYS-1. Immunofluorescence of DNA (left column), H2AK5ac (middle column), and nuclear pore complexes (right column, control) in wild-type (N2), mys-1, xnd-1, and xnd-1;mys-1 hermaphrodite germ lines. The distal end of the germ line is oriented left, and dotted lines mark meiotic entry based on transition zone morphology. All images were taken with identical camera and laser settings and processed identically. Quantification of H2AK5ac intensity was evaluated for the boxed regions and expressed as a ratio of nuclear pore staining (see Materials and Methods). Scale bar, 20 μm. DAPI, 4’,6-diamidino-2-phenylindole; IR, ionizing radiation; NPC, nuclear pore complex.