Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 27;6(12):3913–3925. doi: 10.1534/g3.116.035725

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 McClendon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Altered dynamics of DSBs in xnd-1 are mys-1-dependent. Dissected germ lines were stained with DAPI to visualize DNA and anti-RAD-51 to visualize DSB repair intermediates. The beginning of the transition zone is marked with a solid line, the end with an arrowhead; onset of significant RAD-51 foci accumulation with a dashed line. (A) Kinetics of DSBs are altered in xnd-1 compared to wild-type, with high levels of RAD-51 appearing in the transition zone. DSB accumulation in mys-1 is similar to wild-type, peaking in midpachytene. mys-1 suppresses the formation of early DSBs in xnd-1. (B) Ectopic expression of him-5 cannot suppress the induction of early DSBs in xnd-1. RAD-51 accumulation appears normal in eaIs15 animals (top). xnd-1 is epistatic to eaIs15 and the double mutant shows accumulation of RAD-51 foci in the transition zone. Scale bar, 20 μm. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break.