Figure 1.

Sperm storage dynamics in conspecifically and heterospecifically inseminated D. virilis females. (A) D. virilis female reproductive tract 24 hr after conspecific (left) and heterospecific (right) insemination. Purple and blue arrows indicate the vagina and bursa, respectively. Red arrowheads indicate the paired spermathecae, and yellow arrowheads point to the long seminal receptacle. Sperm can be seen on the left panel as white material in the seminal receptacle, the spermathecal ducts, and lower portion of the bursa (File S5 shows a movie of this sample). The yellow circle on the right panel highlights the magnified region in File S6. (B) Bar graphs of percentage of females that contain none, very few, few, or many sperm at six time-points after conspecific and heterospecific copulation. Percentages are shown for the seminal receptacle (SR; top) and spermathecae (Sp; bottom). hpc, hours post copulation.