Table 3. Effect of paromomycin concentration on transformation efficiency.
| Plasmida | Type | Relative Transformation Efficiency at Different Paromomycin Concentrations (µg/ml) | |||
|---|---|---|---|---|---|
| 10 | 20 | 30 | 40 | ||
| pMO449 | One-promoter (see Figure 1B) | 100 | 26 | 7.1 | 3.2 |
| 100 | 34 | 11 | 3.6 | ||
| 100 | 34 | 11 | 5.0 | ||
| pMO518 | Two-promoter (see Figure 1A) | 100 | 97 | 87 | 83 |
| 100 | 103 | 95 | 90 | ||
| pMO448 | Monocistronic (APHVIII-only) control (see Materials and Methods) | 100 | 101 | 64 | 54 |
| 100 | 93 | 76 | 71 | ||
a
Data are shown from three (pMO449) or two (pMO518 and pMO448) separate transformation experiments using strain CC-124 and various electroporation methods (see Materials and Methods). In each experiment, the numbers of transformants per micrograms of DNA were normalized to the value obtained at 10 µg/ml paromomycin in that experiment.