Figure 1.

Generation of Pp1α-96A mutants using the CRISPR-Cas9 system. (A) Nucleotide sequence of the Pp1α-96A genomic region targeted for mutagenesis. Capital letters, exon sequence; lowercase letters, intron sequence; blue letters, gRNA targets; bold letters, ATG translation start codon; underlined sequence, region shown in panel (B). (B) Nucleotide sequences of control wild-type (WT) and three Pp1α-96A mutant lines. The deleted nucleotides listed are shown as dashes in the sequence. CRISPR, clustered regularly interspaced short palindromic repeats; gRNA, guide RNA.