Skip to main content
. Author manuscript; available in PMC: 2016 Dec 8.
Published in final edited form as: Science. 2013 May 3;340(6132):619–621. doi: 10.1126/science.1234848

Fig. 1. The HD domain protein AtNDX is a regulator of FLC.

Fig. 1

(A) A mutant showing enhanced luciferase activity was isolated and called eoc1 (enhancer of COOLAIR1). Luciferase activity is depicted with false color (blue < green < yellow < red < white). (B) eoc1 maps to AtNDX, which encodes a homeodomain-containing protein with two other conserved domains, NDX-A and NDX-B (fig. S4) (10). eoc1-1 is a point mutation; eoc1-2 and eoc1-4 are T-DNA insertion alleles in Ws and Col-0 back ground, respectively. The pink line indicates the recombinant protein used for EMSA (fig. S9A); aa, amino acids. (C) Endogenous COOLAIR RNA is up-regulated in eoc1; expression data are relative to UBC21, normalized to the wild type. Errors are SEM for three biological replicates.