Table 1.

Two-hybrid interaction between BNIP1 and proteins in the syntaxin 18 complex

Gal4 DNA-binding domain	Gal4 activation domain	β-Galactosidase activitya
Syntaxin 18	BNIP1	+
α-SNAP	BNIP1	+
α-SNAP	BNIP1 L114A	−
BNIP1	RINT-1b	+
BNIP1 L114A	RINT-1	+
ZW10	BNIP1	−
p31	BNIP1	−
Results were recorded as positive if blue color was developed within 4 h on filters.
To analyze the interaction between RINT-1 and BNIP1, the cDNA of RINT-1 was inserted downstream of the Gal4 activation domain because its insertion downstream of the Gal4 DNA-binding domain gave a positive signal without an interacting partner.