Figure 1. H₂O₂ induced apoptosis and up-regulated miR-34a expression in HLE-B3 cells.

A, B: Flow cytometry analysis of the apoptosis induced by H₂O₂. Early apoptotic cells were shown in Q3: lower right quadrant and late apoptotic; Necrotic states were in Q2: upper right quadrant. The numbers of apoptotic cells induced by H₂0₂ were significantly increased. C: Quantitative RT-PCR showed that miR-34a was up-regulated significantly in H₂O₂-treated group. Data were expressed as mean±SD, n=3, ^bP<0.01.