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. 2004 Jul 29;23(16):3325–3335. doi: 10.1038/sj.emboj.7600335

Figure 4.

Figure 4

Regulation of c-MYC, MAD1 and UBF during granulocyte differentiation. (A) Human (HL-60) and mouse (MPRO) granulocytic cell lines were induced to differentiate with ATRA (for 1 and 2 days) and AGN194024 (for 2 and 4 days) respectively as compared to undifferentiated cells (day 0). Protein from cells was analyzed by Western blot for c-MYC, MAD1 and UBF expression. α-Tubulin was used as a protein loading control and ratios of UBF to α-tubulin were calculated. (B) Cells were differentiated as indicated in (A) and UBF mRNA levels were analyzed by Northern blot. Lanes are equally loaded with total RNA as demonstrated by β-actin expression and/or stained for 18S and 28S rRNA. d0=day 0, d1=day 1, d2=day 2 and d4=day 4.