Skip to main content
. 2016 Dec 8;10(12):e0005113. doi: 10.1371/journal.pntd.0005113

Fig 1. SmartAmp2 primer design for β-tubulin mutation (SNP) detection.

Fig 1

Partial sequence of the β-tubulin isotype 1 gene carrying (A) SNP 167A, (B) SNP 198C, and (C) SNP 200A as well as the sequences of primers used for the SmartAmp2 assay for the three SNP positions. The locations of SNPs indicated in bold. The boost primer (BP) was used as the discrimination primer. The folding primer (FP) has a specific sequence (CCTATATATATATAGG) at the 5’ end to allow self-annealing hairpin formation.