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. 2016 Dec 8;10(12):e0005113. doi: 10.1371/journal.pntd.0005113

Fig 5. Genotyping results of N. americanus samples at codon 198 with SmartAmp2 and conventional sequencing.

Fig 5

(A) SmartAmp2 amplification of 198A/C polymorphs using wild-type (WT) and mutant-type (MT)-primer sets. Left, center, and right panels show assay results for homozygous WT (WT/WT), mixed (WT/MT), and homozygous MT (MT/MT) samples, respectively. (B) Conventional sequencing of β-tubulin gene from left to right, WT (A/A), mixed (A/C), and MT (C/C).