Table 2. Precision and accuracy of the antidiabetic agents and internal standards obtained in human plasma by the UPLC-QToF method.
| Compound | Quality control level | Concentration (ng/mL/) | Precision Intraday (RSD%) | Accuracy Intraday (RE%) | Precision Interday (RSD%) | Accuracy Interday (RE%) |
|---|---|---|---|---|---|---|
| Chlorpropamide | LLOQ | 500 | 6.95 | 3.92 | 6.74 | 3.49 |
| LQC | 1000 | 8.20 | 0.54 | 8.01 | -3.35 | |
| MQC | 2500 | 4.61 | -1.26 | 6.13 | 0.82 | |
| HQC | 3500 | 6.87 | 3.50 | 7.40 | 2.90 | |
| DQC | 2500 | 4.74 | 11.11 | 9.24 | 6.19 | |
| Glibenclamide | LLOQ | 125 | 8.03 | 5.76 | 11.02 | 4.96 |
| LQC | 250 | 6.10 | -1.84 | 7.61 | -2.24 | |
| MQC | 1000 | 4.63 | -0.02 | 8.36 | -6.97 | |
| HQC | 1500 | 5.83 | -1.21 | 9.08 | 1.62 | |
| DQC | 10000 | 3.53 | 11.82 | 6.43 | 10.62 | |
| Gliclazide | LLOQ | 50 | 7.64 | 8.80 | 7.01 | 10.02 |
| LQC | 125 | 7.92 | 3.36 | 4.77 | -6.66 | |
| MQC | 500 | 2.81 | -8.52 | 4.46 | -5.29 | |
| HQC | 750 | 2.55 | 3.62 | 3.83 | 1.88 | |
| DQC | 5000 | 4.35 | 6.10 | 5.66 | 3.29 | |
| Glimepiride | LLOQ | 125 | -3.74 | 8.80 | 11.31 | 2.82 |
| LQC | 250 | -5.69 | 3.04 | 7.16 | 0.42 | |
| MQC | 1000 | 5.97 | -1.88 | 6.82 | -6.98 | |
| HQC | 1500 | -1.48 | 7.71 | 5.76 | 3.09 | |
| DQC | 10000 | 5.81 | 1.33 | 7.40 | -4.10 | |
| Metformin | LLOQ | 250 | 10.41 | -2.56 | 11.45 | -11.44 |
| LQC | 500 | 8.86 | -1.96 | 10.71 | 4.73 | |
| MQC | 2000 | 5.02 | 10.6 | 5.19 | 7.31 | |
| HQC | 3000 | 6.25 | -0.01 | 5.75 | -1.15 | |
| DQC | 20000 | 8.92 | -4.98 | 9.17 | -5.08 | |
| Nateglinide | LLOQ | 500 | 1.84 | -2.32 | 4.97 | 2.42 |
| LQC | 1000 | 1.82 | -0.24 | 5.92 | -2.90 | |
| MQC | 2000 | 2.52 | 1.37 | 6.16 | -3.13 | |
| HQC | 3000 | 2.39 | -1.58 | 5.01 | 2.12 | |
| DQC | 20000 | 4.15 | -5.65 | 7.82 | -6.21 | |
| Pioglitazone | LLOQ | 250 | 4.09 | -11.11 | 8.19 | -10.57 |
| LQC | 500 | 5.76 | 8.96 | 7.01 | 6.80 | |
| MQC | 1000 | 4.78 | -0.30 | 8.62 | 6.68 | |
| HQC | 1500 | 5.37 | -3.30 | 6.63 | -2.98 | |
| DQC | 10000 | 5.10 | 5.75 | 7.14 | 3.50 | |
| Rosiglitazone | LLOQ | 125 | 10.25 | -10.82 | 8.86 | -11.50 |
| LQC | 250 | 9.34 | 8.40 | 5.59 | 7.80 | |
| MQC | 500 | 6.13 | 8.08 | 5.97 | 5.91 | |
| HQC | 750 | 9.05 | -3.48 | 6.41 | -2.02 | |
| DQC | 5000 | 3.72 | 0.63 | 4.65 | 2.26 | |
| Vildagliptin | LLOQ | 125 | 6.49 | -2.72 | 11.58 | -2.45 |
| LQC | 250 | 8.50 | -1.12 | 8.02 | 0.14 | |
| MQC | 1000 | 10.53 | 2.02 | 9.09 | 1.27 | |
| HQC | 1500 | 5.78 | 4.64 | 7.83 | 1.09 | |
| DQC | 10000 | 11.90 | -4.50 | 11.52 | -3.32 | |
| Isoniazid (IS) | - | 200 | 7.32 | -0.02 | 8.24 | -0.12 |
| Sulfaquinoxaline (IS) | - | 75 | 11.11 | 6.20 | 11.10 | 1.69 |
LLOQ, lower limit of quantification; LQC, low quality control; MQC, medium quality control; HQC, high quality control; DQC, dilution quality control IS, internal standard; RE%, relative error; RSD%, relative standard deviation. Intraday analysis, n = 5; interday analysis, n = 15.