Table 1. Cell cycle parameters of oriC-FRT mutants with TaqI sitesa.
| TaqI site next to GATC # | Strain No. | Gen. Timeb (min) | Origin/Cellc | Cell massd | Origin/Cell masse | Asyn. Indexc(%) | Frac. of uninitiatedCellsc | Initiation mass |
|---|---|---|---|---|---|---|---|---|
| None (WT) | 2239 | 33.4 | 3.49 | 1 | 1 | 3.9 | 0.20 | 0.39 |
| #1 | 2240 | 34.6 | 3.43 | 0.99 | 0.99 | 12.4 | 0.19 | 0.39 |
| #2 | 2241 | 33.6 | 3.48 | 0.96 | 1.04 | 7.2 | 0.20 | 0.37 |
| #3 | 2242 | 31.6 | 3.33 | 1.05 | 0.91 | 14.5 | 0.19 | 0.40 |
| #4 | 2243 | 33.7 | 3.49 | 0.97 | 1.03 | 4.1 | 0.19 | 0.37 |
| #5 | 2244 | 32.9 | 3.28 | 0.97 | 0.97 | 5.9 | 0.24 | 0.38 |
| #6 | 2245 | 34.1 | 3.34 | 1.01 | 0.95 | 9.6 | 0.22 | 0.39 |
| #7 | 2246 | 32.7 | 3.48 | 1.01 | 0.99 | 8.8 | 0.18 | 0.39 |
| #8 | 2247 | 33.6 | 3.31 | 0.99 | 0.95 | 2.4 | 0.28 | 0.40 |
| #9 | 2248 | 32.7 | 3.40 | 1.06 | 0.92 | 5.0 | 0.22 | 0.42 |
a Other than the generation time, cell cycle parameters were derived from Figure B in S1 File.
bMean generation time from four biological replicates for WT was 33.5 ± 2.2 min. The generation times of the mutants did not vary significantly from that of the WT in pairwise comparisons (p-values 0.18–0.89).
c Origin/cell, asyn. index and the fraction of uninitiated cells were determined from flow cytometry profiles after replication run-out. Origin/cell for WT among four biological replicates was 3.40 ± 0.09. The spread covers the range of values seen for the mutants. Further details are in Materials and Methods.
d Cell mass is the average light scatter from flow cytometric analysis normalized to 1 for WT cells. The values varied about 3% among repeated measurements of the same sample and about 8% among biological replicates.
e Values are normalized to 1 for WT cells.