Table 2. Cell cycle parameters of oriC-zeo mutants with mutated GATC^a.

oriC-zeo with mutated GATC	StrainNo.	Gen. Time(min)	Origin/Cell	Cell Mass	Origin/Cell Mass	Asyn. Index (%)	Frac. of Uninitiat.cells	Initiat. Mass
None (WT)	2073	33.2	3.28	1.0	1.0	4.3	27.5	0.40
#1GATC→GTTC	2901	32.1	3.13	1.07	0.89	11.6	29.7	0.41
#2        ,,     →    ,,	2902	32.8	3.16	1.08	0.89	10.2	29.8	0.41
#3        ,,     →    ,,	2903	32.0	3.22	1.02	0.96	5.3	23.4	0.39
#3*        ,,     →    ,,	2916	31.7	2.99	1.06	0.85	5.7	25.2	0.40
#4        ,,     →    ,,	2904	32.7	3.04	1.06	0.87	6.3	29.9	0.41
#5        ,,     →    ,,	2905	32.2	3.42	1.03	1.01	5.8	22.2	0.39
#6        ,,     →    ,,	2906	32.3	2.94	1.05	0.85	20.2	27.1	0.40
#7        ,,     →    ,,	2907	32.1	3.26	1.03	0.97	7.3	28.4	0.40
#8        ,,     →    ,,	2908	32.0	2.98	1.03	0.88	5.3	30.7	0.41
#9        ,,     →    ,,	2909	34.3	3.06	1.01	0.92	5.1	29.0	0.41
#5–6        ,,     →    ,,	2910	33.2	2.87	1.03	0.85	3.3	23.2	0.39
#8–9        ,,     →    ,,	2911	35.1	2.89	1.07	0.82	5.0	29.7	0.41
#7–9        ,,     →    ,,	2912	35.9	2.95	1.04	0.87	6.0	29.2	0.41
#6–9        ,,     →    ,,	2913	35.1	2.71	1.04	0.80	19.6	26.3	0.40
#6        ,,     →GATG	2914	34.6	3.21	1.06	0.92	5.0	31.4	0.41
#6        ,,     →GAAC	2915	34.7	3.44	1.08	0.97	6.7	19.8	0.38

^a Other than the generation times, cell cycle parameters were derived from Figs 4 and 5.