Table 3. Results of molecular analyses of bat ticks for the presence of piroplasms.
| Ixodesspecies | Tick stage or sex | PCR positive / all analysed ticks | Results of sequencing(length, % identity, sample number) | Bat host of PCR positive ticks# | Location(s) of PCR positive ticks in Fig 1 | Reference sequence | Accession number of sequence in this study (name of isolate) |
|---|---|---|---|---|---|---|---|
| I. ariadnae | larva | 4/26 | Babesia vesperuginis (448 bp, 100%, 4×) | MDAS | 1 | AJ871610 | KU958544 (Ia-Bv-1) |
| nymph | 0/14 | - | - | - | - | - | |
| female | 0/5 | - | - | - | - | - | |
| I. vespertilionis | larva | 3/89 | Babesia vesperuginis (448 bp, 100%, 2×) | ESER, MDAU | 2 | AJ871610 | KU958544 (Ia-Bv-1) |
| Babesia crassa (410 bp, 98.5%, 1×) | RHIP | 3 | KF791205 | KU958546 (Iv-Bcr-1) | |||
| nymph | 0/27 | - | - | - | - | ||
| female | 0/8 | - | - | - | - | ||
| I. simplex | larva | 8/79 | Babesia crassa (410 bp, 98.3%, 1×) | MSCH | 4 | KF791205 | KU958545 (Is-Bcr-1) |
| Babesia venatorum-like (105 bp, 100%, 1×) | MSCH | 2 | KC007118 | KU958553 (Is-Bv-1) | |||
| Babesia canis (420 bp, 100%, 1×) | MSCH | 2 | JF461253 | KU958552 (Is-Bca-2) | |||
| Theileria capreoli (425 bp, 99.5%, 1×) | MSCH | 4 | KJ188219 | KU958547 (Is-Tc-1) | |||
| Theileria orientalis (432 bp, 100%, 4×) | MSCH | 2, 4, 5 | AB668373 | KU958549 (Is-To-1) | |||
| nymph | 4/50 | Babesia crassa (410 bp, 98.5%, 1×) | MSCH | 2 | KF791205 | KU958546 (Iv-Bcr-1) | |
| Babesia canis (420 bp, 100%, 1×) | MSCH | 2 | KC902833 | KU958551 (Is-Bca-1) | |||
| Babesia canis (420 bp, 100%, 2×) | MSCH | 2 | JF461253 | KU958552 (Is-Bca-2) | |||
| female | 1/9 | Theileria sp. OT3 (432 bp, 100%, 1×) | MSCH | 4 | DQ866839 | KU958550 (Is-TOT3-1) |
#Abbreviations: MDAS—Myotis dasycneme, ESER—Eptesicus serotinus, MDAU—M. daubentonii, RHIP—Rhinolophus hipposideros, MSCH—Miniopterus schreibersi