Fig 1. Effect of CCR2 deficiency in resident cells of the CNS or in the hematopoietic system on mouse survival rates during HSE.

Eight-week old recipient wild-type (WT) C57BL/6 and CCR2^-/- mice conditioned with irradiation were transplanted with bone marrow cells harvested from CCR2^-/- (CCR2^-/-→WT) and WT (WT→CCR2^-/-) animals, respectively. Total body CCR2^-/- and WT mice were used as controls. Sixteen-week old WT, CCR2^-/-, CCR2^-/-→WT and WT→CCR2^-/- mice (n = 14 mice per group) were infected intranasally with HSV-1 (1.2x10⁶ plaque forming units (PFU) in 20 μl minimal essential medium). Mice were examined carefully twice a day for 20 days, and two obvious signs of sickness or a ≥ 20 % weight loss were considered as endpoints for sacrifice. Survival curves were analysed using a log-rank (Mantel-Cox) test. Statistically significant results between groups are indicated as follows: *, P<0.05; **, P<0.01.