Fig 2. Impact of CCR2 deficiency in resident cells of the CNS or in the hematopoietic system on the control of viral replication during HSE.
Wild-type (WT) C57BL/6, CCR2-/-, CCR2-/-→WT and WT→CCR2-/- mice were sacrificed prior to (day 0) and on days 4, 6, 8 and 10 following intranasal infection with 1.2x106 PFU of HSV-1. Viral titers were measured in brain homogenates by a standard plaque assay on Vero cells. Results are reported as PFU/μL of brain homogenates and represent the means ± standard deviations of 4 to 5 mice per group at each time point. Statistical analyses were performed using a one-way analysis of variance (ANOVA) with Tukey’s multiple comparison post-test. Statistically significant results compared to those for the WT group are indicated as follows: *, P<0.05; **, P<0.01; ***, P<0.001. ND, not detected.