Table 2.
Membrane functional changes as a function of growth rate and cultivation technique
| FUNCTION 2 | Continuous cultivation | Fed-batch cultivation | Comment | ||||
| Parameter | Low μ | Min/Max | High μ | Low μ | Min/Max | High μ | |
| Total protein production (per CDW) | R | High | High | R | High | High | Yield drops from the theoretical 0.5 to approx. 0.4 |
| Specific protein production (per tot prod.) | R | High | High. | R | High | High | Model substance: periplasmic β-lactamase |
| Cell lysis (%) | 8 | 1 | 1 | 1 | <1 | <1 | |
| Effect of sonication (% release of specific protein) | 56 | 78 | 73 | 57 | 80 | 85 | Result of 1 min sonication |
| Endotoxin release (% of CDW) | 1 | 1 | 1 | <1 | <1 | <1 | |
| Periplasmic leakage | 1.5 | 19 | 7 | 10 | 10 | 9 | |
2Summary of the structural differences in continuous and fed-batch cultivation as a function of growth rate. Results compared to data from Shokri et al 2002. A high growth rate refers to μ = 0.6 h-1 and a low growth rate to μ = 0.05 h-1.