Table 3. Oxidative stress assessment in left ventricle (LV) and plasma in sedentary control (SC), trained control (TC), sedentary fructose (SF) and trained fructose (TF) groups.
| SC | TC | SF | TF | |
|---|---|---|---|---|
| LV | ||||
| Lipoperoxidation | ||||
| TBARS (μmol/mg protein) | 5.36 ± 0.40 | 4.85 ± 0.82 | 4.33 ± 0.35 | 2.95 ± 0.20 *#† |
| CL (cps/mg protein) | 5846 ± 215 | 5795 ± 186 | 6800 ± 296*# | 5255 ± 212 † |
| Protein Oxidation (nmol/mg protein) | 4.71 ± 0.51 | 3.85 ± 0.45 | 3.22 ± 0.34 | 2.89 ± 0.37 * |
| CAT (nmol/mg protein) | 0.26 ± 0.02 | 0.42 ± 0.03* | 0.31 ± 0.03 | 0.33 ± 0.03 |
| SOD (USOD/mg protein) | 14.91 ± 0.54 | 14.26 ± 0.88 | 11.98 ± 0.75* | 12.61 ± 0.94 |
| GPx (μmol/min/mg protein) | 0.029 ± 0.001 | 0.035 ± 0.008 | 0.029 ± 0.002 | 0.035 ± 0.001 |
| GSH/GSSG | 11.95 ± 0.52 | 11.25 ± 0.66 | 12.94 ± 0.53 | 16.17 ± 0.33 *#† |
| PLASMA | ||||
| Lipoperoxidation | ||||
| TBARS (μmol/mg protein) | 0.10 ± 0.01 | 0.10 ± 0.01 | 0.19 ± 0.03 *# | 0.19 ± 0.02 *# |
| Protein Oxidation (nmol/mg protein) | 1.45 ± 0.08 | 1.49 ± 0.11 | 3.30 ± 0.09 *# | 2.75 ± 0.18 *#† |
| SOD (USOD/mg protein) | 1.00 ± 0.03 | 0.98 ± 0.05 | 0.95 ± 0.03 | 0.91 ± 0.04 |
Data are reported as mean ± SEM. Antioxidant enzymes: SOD = Superoxide dismutase; GPx = Glutathione peroxidase; CAT = Catalase.
* p<0.05 vs SC
# p<0.05 vs TC
† p<0.05 vs SF