Table 4.
Summary of RT-PCR details and the numbers of samples in each analysis. Analysis of hMLH1, IL-15 and Caspase2 restricted to smaller numbers due to limited availability of high-quality RNA.
| Gene name | GENBANK NUMBER | Chemistry (Applied Biosystems, Warrington, UK) | Sample Numbers (n) | |
| MSS | MSI-H | |||
| hMLH1 | NM_000249 | High-Capacity cDNA Archive Kit + TaqMan Universal PCR Master Mix (with AmpErase UNG) | 12 | 16 |
| TP53 | NM_000546 | TaqMan® EZ-RT-PCR Kit | 26 | 27 |
| HSP-70 kD 1B | NM_005346 | TaqMan® EZ-RT-PCR Kit | 26 | 28 |
| HSP-110 | NM_014278 | TaqMan® EZ-RT-PCR Kit | 26 | 28 |
| IL-18 | NM_001562 | TaqMan® EZ-RT-PCR Kit | 24 | 28 |
| IL-8 | NM_000584 | TaqMan® EZ-RT-PCR Kit | 26 | 28 |
| IL-15 | NM_000585 | High-Capacity cDNA Archive Kit + TaqMan Universal PCR Master Mix (with AmpErase UNG) | 12 | 16 |
| Granulysin | NM_006433 | TaqMan® EZ-RT-PCR Kit | 26 | 27 |
| Caspase 2 | NM_001224 | TaqMan® EZ-RT-PCR Kit | 21 | 18 |