Figure 3.
Cell viability and vitality assay on SH-SY5Y-tau cells. The dash-circled cell was punctured by nFTP once every hour for 12 h. The solid-circled cell was punctured by a microscale fiber. (a) Cell viability/cytotoxicity (L3224, Invitrogen) assay. Green-fluorescence indicated active esterase condition. Red-fluorescence indicated loss of cell membrane integrity. (b) Cell vitality (L34951, Invitrogen) assay. Green-fluorescence indicated loss of cell membrane integrity. Red-fluorescence indicated active metabolic condition.