Table 2.
Comparison of Cre strains for conditional deletion of genes in dendritic cells
| Strain | Cells Affected | Caveats |
|---|---|---|
| Itgax-cre (BAC) (Caton et al., 2007) | ~100% of cDCs, pDCs, alveolar macrophages, ~70% red pulp macrophages, 20–40% monocytes, marginal zone macrophages, peritoneal macrophages, ~5–12% T, B, NK cells | Germline deletion possible |
| Itgax-cre (Transgene) (Stranges et al., 2007) | cDCs, pDCs | Deletion in other cell types was not analyzed, most likely similar to other Itgax- cre strain |
| Clec9a-cre (Schraml et al., 2013) | ~100% of cDC1s, ~50% of cDC2s, ~20% of pDCs | Begins deletion at CDP stage, low or transient expression may affect deletion efficiency in cDC2s and pDCs |
| Zbtb46-cre (Loschko et al., 2016b) | 65–95% cDCs, <10% in all other cell types | Deletion efficiency variable depending on floxed allele |
| Cd207-cre (Zahner et al., 2011) | Langerhans cells, Langerin+ cDC1s in dermis, skin-draining LNs, and lung | |
| Xcr1-cre (Ohta et al., 2016) | cDC1s | |
| Siglech-cre (Puttur et al., 2013) | ~30% of pDCs, ~2% of other lymphoid cells | Incomplete deletion in pDCs Deletion in marginal zone macrophages untested but possible |