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Lanes 1, 4, and 7 are kinases without any substrate. CDK8 and CDK9 have background bands that resulted from their autophosphorylation. CDK7 produced two bands with GST-CTD, the low mobility band corresponds to the hyperphosphorylated form (IIo), and the higher mobility band, the hypophosphorylated IIa. CDK8 and CDK9 produced only the IIa form.
