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Western blots showing gel filtration elution profiles of p68, p72 and HDAC1. p68, p72 and HDAC1 in the fractions were detected by western blotting using appropriate antibodies. Note that the antibody raised against p72 also recognises p82 and cross-reacts with NFAR-2 [15]. All lysates had been treated with DNase and RNase prior to gel filtration. The void volume and elution position of the Pharmacia FPLC size markers are indicated, as are molecular weight markers (in kDa).
