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. 2016 Nov;177(1-3):18–27. doi: 10.1016/j.schres.2016.04.015

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© 2016 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Western blotting with ab166608 antibody. Panel A: Immunoblotting profile in membranes. Lane 1: Wild-type mouse brain. 2: Grm2^−/−/3^−/− double knockout mouse brain. 3: Wild-type mouse brain. 4: Grm3^−/− knockout mouse brain. 5: Rat brain. 6: Human superior temporal cortex. 7: Transfected HEK293T/17 cells. Lanes 1, 3, and 5–7 show the selective detection of two immunoreactive bands at the molecular weights predicted for monomeric and dimeric mGlu3 (~ 100 kDa and ~ 200 kDa respectively). The bands are specific to mGlu3 as judged by their absence in Grm2^−/−/3^−/− and Grm3^−/− mice (lanes 2 and 4). Panel B: Staining with ab166608 antibody is concentrated in the membrane fraction (lane 8) relative to the cytosol (lane 9); note the latter also lacks a band corresponding to the dimer. Numbers are molecular weight (kDa).