Fig. 6.

Phagocytosis is enhanced in Brown Swiss macrophages compared to Holstein Friesian macrophages.

(A) MØ derived from PBMC isolated from BS and HF cattle were incubated with Alexa-594 labelled S.a bioparticles for up to 60 min before fixing with 4% PFA to suspend phagocytosis. Cells were analysed with BD FACS Calibur recording 10,000 events using Cell Quest Pro. Data are presented corrected against MFI at 0 min and post analysed with FlowJo v10 and GraphPad Prism using unpaired students t-test with Bonferroni post-hoc correction applied. (B) MØ were derived from PBMC isolated from BS and HF and adhered overnight prior to exposure to S. typhimurium (10CFU) for 1 h. Supernatants were collected, cells were washed and culture was continued in the presence of Gentamycin for 24 h. Supernatants (1 h and +24 h) and lysed MØ preparations where serially diluted 10-fold and plated for counting. Data were collated and post analysed using GraphPad Prism using unpaired students t-test with Bonferroni post-hoc testing.