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. 2016 Dec 9;7:581. doi: 10.3389/fimmu.2016.00581

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Copyright © 2016 Flies, Lyons, Corcoran, Papenfuss, Murphy, Knowles, Woods and Hayball.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry (IHC) lung tissue with devil facial tumor 2 (DFT2) metastases. IHC images of lung tissue from TD467. DFT2 metastases are not visible in the images shown here. The scale bar in the upper left of each ×10 image is 200 μm and the ×40 images are 50 μm. The lungs contained low numbers of lymphocytes with positive cytoplasmic staining for PD-1, but the lymphocytes are predominantly peribronchiolar and not near tumor metastases. PD-L1 was not detected on tumor cells and was rare on non-tumor cells.