Figure 4.

PD-L1 mRNA expression in PBMCs and devil facial tumor 1 (DFT1) cells. Expression of PD-L1 in concanavalin A-stimulated PBMCs and DFT1 C5065 cells with and without IFN-γ treatment. The mRNA was reverse transcribed and expression of PD-L1 was tested via PCR. GAPDH was used as a reference gene and β2-microglobulin (B2m) was used as a positive control. The unstimulated tumors express only low levels of B2m and PD-L1 transcripts, but both B2m and PD-L1 expression increases following stimulation with IFN-γ. NTC, no template control. The rightmost lane in the gel contains the 1 kb DNA ladder.