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. 2016 Dec 9;7:581. doi: 10.3389/fimmu.2016.00581

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Copyright © 2016 Flies, Lyons, Corcoran, Papenfuss, Murphy, Knowles, Woods and Hayball.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry (IHC) of devil lymph nodes (LN). IHC of LN from a devil facial tumor 1 (DFT1) + devil (TD 374). The scale bar in the upper left of each ×10 image is 200 μm and the ×40 images are 50 μm. No tumor metastases were observed in the LN shown in this figure. PD-1 is expressed on 0–10% of lymphocytes in the cortical germinal centers but less than 1% of non-germinal center lymphocytes. PD-L1 expression is distributed throughout the cortex of the LN, but was detected on less than 1% of lymphocytes (at ×40 objective).