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. 2016 Dec 9;7:581. doi: 10.3389/fimmu.2016.00581

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Copyright © 2016 Flies, Lyons, Corcoran, Papenfuss, Murphy, Knowles, Woods and Hayball.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry (IHC) devil facial tumor 1 (DFT1) tissue. IHC images of DFT1 tissue sections from TD 512 using the ×10 and ×40 objectives. The scale bar in the upper left of each ×10 image is 200 μm and the ×40 images are 50 μm. The bottom row is stained with α-Periaxin, which is used to identify DFT1 cells. PD-L1 was occasionally detected on tumor cells but was more commonly detected in low numbers of lymphocytes, plasma cells, and macrophages within the fibrovascular stroma supporting multiple lobules within the tumor.