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. 2016 Dec 9;6:171. doi: 10.3389/fcimb.2016.00171

Figure 5.

Figure 5

Effects of Nup98 on Th17 cell differentiation in AVMC. CVB3 infected CD4+ T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, anti-IL-4 and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4+ T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4+ T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 vs. pcDNA3.1 and NC groups. NC: Nonsilencing siRNA sense.