Table 1.
Grouping of the PA gene carrier BAC clones screened by PCR from Brassica juncea.
| Gene Type | Arabidopsis homolog | Primer pair used | Predicted gene | Cloned gene | No. BACs | BAC clone(s) carrying the gene |
|---|---|---|---|---|---|---|
| Structural | TT4/CHS | STT4 | g125911 | BjuTT4-1 | 5 | ZBjuH038D07, ZBjuH052L16, ZBjuH187G14, ZBjuH187G15, ZBjuH187H15 |
| – | BjuTT4-2 | 14 | ZBjuH036L22, ZBjuH062E10, ZBjuH068P04, ZBjuH090C04, ZBjuH103N17, ZBjuH115L06, ZBjuH117A13, ZBjuH125A20, ZBjuH130C13, ZBjuH167H05, ZBjuH167H11, ZBjuH167H12, ZBjuH175I06, ZBjuH187A11 | |||
| g94262 | BjuTT4-3 | 16 | ZBjuH037O10, ZBjuH040M24, ZBjuH042E09, ZBjuH054M24, ZBjuH058B18, ZBjuH102A19, ZBjuH103N21, ZBjuH110J17, ZBjuH111B11, ZBjuH119C13, ZBjuH124I11, ZBjuH129K08, ZBjuH139O13, ZBjuH143K02, ZBjuH162N03, ZBjuH165A05 | |||
| g160192 | BjuTT4-4 | 5 | ZBjuH031A21, ZBjuH031B12, ZBjuH036O12, ZBjuH048I18, ZBjuH095E01 | |||
| g112186 | BjuTT4-5 | 2 | ZBjuH044O21, ZBjuH053C09 | |||
| g134422 | BjuTT4-6 | 2 | ZBjuH053C08, ZBjuH115L05 | |||
| – | BjuTT4-7 | 4 | ZBjuH049I15, ZBjuH049J15, ZBjuH090K23, ZBjuH121I20 | |||
| TT5/CHI | STT5 | g10826 | BjuTT5-1 | 1 | ZBjuH186N11 | |
| g147891 | BjuTT5-2 | 1 | ZBjuH181K10 | |||
| g94675 | BjuTT5-3 | 10 | ZBjuH027P19, ZBjuH036L22, ZBjuH041J15, ZBjuH058J20, ZBjuH066I18, ZBjuH066O10, ZBjuH080G05, ZBjuH156B18, ZBjuH158J20, ZBjuH177D05 | |||
| g153768 | BjuTT5-4 | 5 | ZBjuH096N21, ZBjuH106O08, ZBjuH108L09, ZBjuH119I24, ZBjuH122O18 | |||
| TT6/F3H | STT6 | g93144 | BjuTT6-1 | 7 | ZBjuH020C14, ZBjuH048G02, ZBjuH048M11, ZBjuH058K21, ZBjuH120F22, ZBjuH165E24, ZBjuH181K08 | |
| g230814 | BjuTT6-2 | 5 | ZBjuH058P02, ZBjuH059D03, ZBjuH076G06, ZBjuH087J23, ZBjuH144L06 | |||
| g34078 | BjuTT6-3 | 1 | ZBjuH031F14 | |||
| g58779 | BjuTT6-4 | 8 | ZBjuH022O18, ZBjuH025L04, ZBjuH047N11, ZBjuH088F14, ZBjuH095M08, ZBjuH106B12, ZBjuH132K03, ZBjuH132P11 | |||
| g51817 | BjuTT6-5 | 6 | ZBjuH106N13, ZBjH131P01, ZBjuH143I07, ZBjuH146J13, ZBjuH149K20, ZBjuH171M24 | |||
| TT7/F3'H | STT7 | g118579 | BjuTT7-1 | 9 | ZBjuH012H01, ZBjuH025M21, ZBjuH045C24, ZBjuH063G22, ZBjuH095P11, ZBjuH105C09, ZBjuH156A19, ZBjuH159L04, ZBjuH175L17 | |
| g105339/ g105340 | BjuTT7-2 | 4 | ZBjuH080O14, ZBjuH081G21, ZBjuH092C04, ZBjuH153O14 | |||
| TT3/DFR | SDFR | g119544 | BjuTT3-1 | 7 | ZBjuH029J10, ZBjuH043G11, ZBjuH118M13, ZBjuH119K03, ZBjuH157O03, ZBjuH157P04, ZBjuH184C12 | |
| g127201 | BjuTT3-2 | 3 | ZBjuH134O05, ZBjuH175D09, ZBjuH183H13 | |||
| TT18/ANS | STT18 | g16568 | BjuTT18-1 | 4 | ZBjuH054O02, ZBjuH091D16, ZBjuH181K13, ZBjuH187D05 | |
| g178347 | BjuTT18-2 | 3 | ZBjuH020C14, ZBjuH181I12, ZBjuH181K08 | |||
| g86816 | BjuTT18-3 | 3 | ZBjuH091K10, ZBjuH097N14, ZBjuH178L19 | |||
| g114026 | BjuTT18-4 | 5 | ZBjuH054H16, ZBjuH093H16, ZBjuH177N08, ZBjuH182I21, ZBjuH187H15 | |||
| ANR | SANR | g97466 | BjuANR-1 | 3 | ZBjuH022P08, ZBjuH082J01, ZBjuH123C06 | |
| g177273 | BjuANR-2 | 2 | ZBjuH148I16, ZBjuH165M04 | |||
| g228640 | BjuANR-3 | 4 | ZBjuH071P08, ZBjuH116E04, ZBjuH116I23, ZBjuH185I01 | |||
| g19699 | BjuANR-4 | 1 | ZBjuH034P21 | |||
| TT10 | STT10-1 | g60604 | BjuTT10-1 | 6 | ZBjuH003E23, BjuH033G01, ZBjuH048L18, ZBjuH057G09, ZBjuH083G18, ZBjuH152B03 | |
| STT10-2 | g161120 | BjuTT10-2 | 9 | ZBjuH006C17, ZBjuH019G11, ZBjuH055H16, ZBjuH107M03 ZBjuH121G03, ZBjuH126F02, ZBjuH140A18, ZBjuH140E23, ZBjuH144O05, | ||
| STT10-1 | g169945 | BjuTT10-3 | 11 | ZBjuH021A16, ZBjuH024M11, ZBjuH025E16, ZBjuH037G20 ZBjuH066O13, ZBjuH080H07, ZBjuH084L21, ZBjuH092L15, ZBjuH101B03, ZBjuH144O03, ZBju155P16 | ||
| STT10-2 | g6758 | BjuTT10-4 | 1 | ZBjuH176D10 | ||
| Regulatory | TT1 | STT1 | g65737 | BjuTT1-1 | 4 | ZBjuH021J20, ZBjuH036J21, ZBjuH157B22, ZBjuH180A05 |
| g10440 | BjuTT1-2 | 3 | ZBjuH097N03, ZBjuH147E23, ZBjuH176G24 | |||
| TT2 | STT2 | g27300 | BjuTT2-1 | 2 | ZBjuH085H24, ZBjuH137N11 | |
| g136881 | BjuTT2-2 | 7 | ZBjuH028M22, ZBjuH034J15, ZBjuH061O14, ZBjuH068M24, ZBjuH135H01, ZBjuH149C17, ZBjuH172K23 | |||
| TT8 | STT8-1 | g113056 | BjuTT8-1 | 5 | ZBjuH004L18, ZBjuH038M05, ZBjuH068D18, ZBjuH122I23, ZBjuH173H05 | |
| STT8-2 | g109603 | BjuTT8-2 | 3 | ZBjuH005J18, ZBjuH033E04, ZBjuH036F18 | ||
| TT16 | STT16-1 | g141603 | BjuTT16-1 | 2 | ZBjuH051G23, ZBjuH130K12 | |
| STT16-1 | g157583 | BjuTT16-2 | 6 | ZBjuH046H18, ZBjuH070H21, ZBjuH082B14, ZBjuH099A21, ZBjuH153H13, ZBjuH171M11 | ||
| STT16-2 | g150784 | BjuTT16-3 | 2 | ZBjuH091L03, ZBjuH163M05 | ||
| STT16-2 | – | BjuTT16-4 | 4 | ZBjuH057K05, ZBjuH057K06, ZBjuH098G12, ZBjuH160B19 | ||
| STT16-1 | g231621 | BjuTT16-5 | 7 | ZBjuH061F23, ZBjuH064O06, ZBjuH070C13, ZBjuH094F17, ZBjuH094N07, ZBjuH131N02, ZBjuH135M11 | ||
| STT16-1 | g170816 | BjuTT16-6 | 11 | ZBjuH013K01, ZBjuH030F17, ZBjuH049C21, ZBjuH077C18, ZBjuH077C23, ZBjuH081M09, ZBjuH093J03, ZBjuH142O22, ZBjuH144E18, ZBjuH152O04, ZBjuH171A06 | ||
| TTG1 | STTG1 | g228836 | BjuTTG1-1 | 2 | ZBjuH030O08, ZBjuH130K10 | |
| g55489 | BjuTTG1-2 | 6 | ZBjuH129A18, ZBjuH135B10, ZBjuH140O11, ZBjuH182K06, ZBjuH185M13, ZBjuH185M14 | |||
| TTG2 | STTG2 | g112447 | BjuTTG2-1 | 1 | ZBjuH088A24 | |
| g173809 | BjuTTG2-2 | 3 | ZBjuH101A24, ZBjuH131A11, ZBjuH170G21 | |||
| g118314 | BjuTTG2-3 | 13 | ZBjuH025O05, ZBjuH032N08, ZBjuH039D04,ZBjuH063L13, BjuH065B18, ZBjuH065I18, ZBjuH066D01, ZBjuH067B22, ZBjuH076O21, BjuH135G23, ZBjuH174G04, ZBjuH174O02, ZBjuH184O06 | |||
| g156630 | BjuTTG2-4 | 5 | ZBjuH028C13, ZBjuH043G17, ZBjuH77P08, ZBjuH147A11, ZBjuH162F23 | |||
| Transporter | TT12 | STT12 | g29228 | BjuTT12-1 | 4 | ZBjuH046J03, ZBjuH047J16, ZBjuH148K24, ZBjuH148O16 |
| g146440 | BjuTT12-2 | 3 | ZBjuH124J12, ZBjuH125I12, ZBjuH150E09 | |||
| TT19 | STT19 | g72809 | BjuTT19-1 | 13 | ZBjuH006M03, ZBjuH037H06, ZBjuH061A09, ZBjuH064M21, ZBjuH066G20, ZBjuH092A06, ZBjuH093G08, ZBjuH095N01, ZBjuH140M06, ZBjuH161G15, ZBjuH165B07, ZBjuH172L17, ZBjuH185D12 | |
| g159509 | BjuTT19-2 | 6 | ZBjuH062L17, ZBjuH120J17, ZBjuH143N08, ZBjuH170C22, ZBjuH179C03, ZBjuH181C15 | |||
| g118434 | BjuTT19-3 | 5 | ZBjuH021A20, ZBjuH070G12, ZBjuH122M08, ZBjuH164K02, ZBjuH168M17 |
The BAC clone in bold was used to sequence full-length sequence of the gene.