Figure 4.

Temporal expression of Rhopr-IGF in the CNS, fat body, and dorsal vessel of R. prolixus throughout the fifth instar. Insects were dissected immediately and 1 week after ecdysis into fifth instars, and then 2 weeks after ecdysis one group of insects was fed on blood and then transcript expression was assayed at 3 h, 1, 3, 7, 14, and 21 days post-feeding. Five insects were dissected at each time point and the expression was quantified by real time qPCR in the CNS, the fat body, and the dorsal vessel. Results are shown as means of three biological replicates plus standard error. ^*Indicates statistically significant differences between each time point and the time point used as reference (3 h after feeding) and the P-values are indicated.