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. 2016 Dec 9;7:568. doi: 10.3389/fimmu.2016.00568

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Copyright © 2016 Hjortø, Larsen, Steen, Daugvilaite, Berg, Fares, Hansen, Ali and Rosenkilde.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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3D chemotaxis of human dendritic cells (DCs) in response to CCL19 and CCL21. Human DCs naturally expressing CCR7 were used for the 3D chemotaxis assay. In this assay, a collagen filled channel with the cells is in contact with a source and sink reservoir on either side, causing the cells to experience a linear gradient as chemokine gradually diffuses from source to sink. (A) Column diagrams showing the directional migration of DCs in response to either 10 nM (left) or 100 nM (right) CCL19 or CCL21 source concentrations. The values are calculated as chemotactic index (CI) in the MATLAB software. (B) Spider diagrams depicting the migration pattern in response to the indicated concentrations of the chemokines. Statistical significance was calculated using unpaired t test. NS, not significant; *P < 0.05 (n = 3).