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. 2016 Dec 9;7:568. doi: 10.3389/fimmu.2016.00568

Figure 2.

Figure 2

ERK1/2 activation in human dendritic cells in response to CCL19 and CCL21. Human DCs with natural CCR7 expression were used for the ERK1/2 activation. The percentage of phosphorylated ERK1/2 was calculated as described by the manufacturer (Meso Scale Discovery, MD, USA) as % Phosphoprotein = [(2 × Phospho-signal)/(Phospho-signal + Total signal)] × 100. The pERK1/2 data are normalized to buffer and displayed as fold ERK1/2 activation over buffer control. (A–C) The effect of 1 nM (A), 10 nM (B), or 100 nM (C) of CCL19 and CCL21. (D) The effect of PTX (10 μg/ml) on ERK1/2 activation by CCL19 and CCL21. (E) Timely effect of 10 nM and (F) 100 nM CCL19 and CCL21 on DC ERK1/2 phosphorylation. (G) The effect of neuraminidase (NA) treatment of DCs on ERK1/2 activation by CCL21 and (H) by CCL19. Statistical significance was calculated using unpaired t test. NS, not significant; *P < 0.05, **P < 0.01 (n = 3–6).