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. 2016 Dec 9;7:568. doi: 10.3389/fimmu.2016.00568

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Copyright © 2016 Hjortø, Larsen, Steen, Daugvilaite, Berg, Fares, Hansen, Ali and Rosenkilde.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CCL21 binds to the surface of human DCs and forms discrete puncta; a feature not matched by tailless-CCL21. Fluorescence microscopy pictures of DCs incubated (A) with100 nM CCL21, (B) 100 nM tailless-CCL21, or (C) in the absence of ligand obtained on LSM 780 confocal microscope using 63× oil-objective (ligands were stained with Alexa 488 and the cell nucleus visualized with Hoecst DNA staining). (D) Zoom in on DC incubated with 100 nM CCL21 to visualize that anti-CCL21 staining followed the villi-like surface of non-adherent DCs. (E) Visualization of two different types of CCL21 puncta. (F) The anti-CCL21 antibody also recognized tailless-CCL21.