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Diagram of the modified PICS bag. Layers consisted of: (Layer 1) 1 kg of conditioned maize; (Layer 2) three small mesh bags containing A. flavus-colonized maize and three small mesh bags containing non-colonized maize; (Layer 3) 3 kg of conditioned maize; (Layer 4) three small mesh bags containing A. flavus-colonized maize and three small mesh bags containing non-colonized maize; and (Layer 5) 1 kg of conditioned maize.
