Fig. 3.

Intact cell photolabeling of NPC2-myc-his with [³H]AC. CT43 cells transiently transfected with WT or with various mutant NPC2-myc-his constructs as indicated were photolabeled by using [³H]AC according to procedures described in Materials and Methods. The IPs were with mouse monoclonal anti-c-myc antibody or with control mouse IgG as indicated. RL, radioluminography; WB, Western blotting. The Western blotting was with rabbit polyclonal anti-NPC2 antibodies. Results are representative of two experiments.