Fig. 1.
Affinity purification of Mtb ICD-1 and Mtb ICD-2. His-tagged recombinant protein was purified by nickel column chromatography under native conditions and stained with Coomassie blue after 10% SDS/PAGE. Lanes: 1 and 2, Mtb ICD-2; M, protein molecular mass markers (200, 116, 97, 66, 45, 31, and 21.5 kDa); 3 and 4, Mtb ICD-1.