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. 2004 Aug 10;101(34):12694–12699. doi: 10.1073/pnas.0403127101

Fig. 4.

Fig. 4.

RNA 2′-O-ribose methylation blocks clearage by coronavirus nsp15. dsRNA substrates were produced by annealing RNA8 to the complementary 5′-[32P]-labeled RNA3 or its 2′-O-ribose-methylated derivative, RNA9. Substrates were incubated with SARS-CoV (SCoV) and HCoV-229E (HCoV) MBP-nsp15, respectively, or inactive control proteins. Reaction products were analyzed by denaturing PAGE and autoradiography. Lanes 1 and 6, reactions without protein; lanes 2 and 7, reactions with HCoV-229E MBP-nsp15_H6360A; lanes 3 and 8, reactions with HCoV-229E MBP-nsp15; lanes 4 and 9, reactions with SARS-CoV MBP-nsp15_H6678A; lanes 5 and 10, reactions with SARS-CoV MBP-nsp15.