Table 2. Sequences of sgRNAs used in this study.

All sgRNAs are 20 nucleotides long. Those without a 5' G nucleotide had one added for efficient U6 promoter transcription to make a 21 nucleotide sequence.

sgRNA name	Experiment	Sequence (5’ ➔ 3’)
sgRNA A	Knock-out of MeCP2	AGAAGCTTCCGGCACAGCCG
sgRNA B	Knock-out of MeCP2	CGCTCCATCATCCGTGACCG
sgRNA 1	Knock-in of R306C-MECP2	CCATCAAGAAGCGCAAGACC
sgRNA 2	Knock-in of R306C-MECP2	GACGGTCAGCATCGAGGTCA
sgRNA 3	Knock-in of R111G-MECP2	GGACACGGAAGCTTAAGCAA
sgRNA 4	Knock-in of R133C-MECP2	AAAAGCCTTTCGCTCTAAAG
sgRNA 5	Knock-in of T158M-MECP2	GATTTTGACTTCACGGTAAC
sgRNA 6	Knock-in of T158M-MECP2	ATTTTGACTTCACGGTAACT
sgRNA 1	Knock-in of D252H-EEF1A2	GCTTGTCCGTGGGGCGCGTG
sgRNA 2	Knock-in of D252H-EEF1A2	CAGGACGTGTACAAGATTGG
sgRNA C	Knock in of mCherry tag into MECP2	TTAGCTGACTTTACACGGAG