Figure 7. Analytical gel chromatography of MmNAGS-K and XcNAGS-K.

(A) and (C) MmNAGS-K and XcNAGS-K that were allowed to equilibrate overnight at indicated protein concentrations with and without L-arginine. (B and D) MmNAGS-K and XcNAGS-K were diluted immediately before loading onto column and did not equilibrate with L-arginine prior to loading onto column. The top panels show a semi-logarithmic plot of molecular mass vs. elution volume of size and molecular weight standard proteins. Lower panels show absorption at 280 nm as a function of elution volume and protein concentration. Blue - elution profiles in the absence of L-arginine. Orange – elution profiles in the presence of 1 mM L-arginine.