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. 2016 Dec 9;6:38031. doi: 10.1038/srep38031

Figure 1. Circular dichroism spectra of PCP in TRIS-HCl with and without DTT as a function of temperature.

Figure 1

(A) CD spectra obtained for the same sample at 8 temperatures ranging from 25 to 95 °C, in 10 °C steps, as represented by each curve. A characteristic α-helical profile is depicted, with minimums in 208 and 222 nm. (B) The same conditions were analyzed in the presence of 2 mM DTT. PCP secondary structure content was significantly altered by addition of DTT and even more so after subsequent heating.