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. 2016 Dec 9;6:38694. doi: 10.1038/srep38694

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) The typical epithelial morphologies of hiHeps and PHHs, as determined by light phase contrast microscopy. (B) The mRNA expression levels of BA synthases (i.e., CYP7A1, CYP8B1, and CYP27 A1) and the upstream nuclear factors (i.e., FXR, CAR, and PXR) in hiHeps. as determined by qRT-PCR. The data are expressed as the mean ± SD (n = 3). *p < 0.05 relative to PHHs. (C) Comparison of the protein expression levels of CYP7A1 and FXR among hiHeps, PHHs and SCHHs by western blotting (left) and gray intensity analysis (right). β-ACTIN was used as a reference control.