Table 1. Sequence of primers and probes used in this study.

PCR primers/Probes	Sequence	Target	Amplicon Size	Reference
ZIKV 1086	5′ CCG CTG CCC AAC ACA AG 3′	E (envelope gene)	77 bp	10
ZIKV 1162c	5′ CCA CTA ACG TTC TTT TGC AGA CAT 3′
ZIKV 1107-FAM probe	5′ FAM- AGC CTA CCT TGA CAA GCA GTC AGA CAC TCA A-BHQ-1 3′
RPA ZV-F	5′ CAT ATA CTT GGT CAT GAT ACT GCT GAT TGC C 3′	prM (premembrane gene)	88 bp	This study
RPA ZV-R	5′ CAT ACC TTC CAC AAA GTC CCT ATT GCT GAC 3′
RPA exoZV-FAM probe	5′ TAC CTT CCA CAA AGT CCC TAT TGC TGA CTC C-T (FAM)-dSpacer-T(BHQ-1)-GC ACC TGA TGC TGT ATG C-Spacer C3 3′